n/a
Abstract Title:

Risk reduction of ethyl acetate fraction of Empetrum nigrum var. japonicum via antioxidant properties against hydrogen peroxide-induced cell damage.

Abstract Source:

J Toxicol Environ Health A. 2009 ;72(21-22):1499-508. PMID: 20077224

Abstract Author(s):

Ki Cheon Kim, Kyoung Ah Kang, Rui Zhang, Mei Jing Piao, Young Jun Heo, Sungwook Chae, Gi Young Kim, Ji Young Moon, Byoung Sam Yoo, Jin Won Hyun

Article Affiliation:

Ki Cheon Kim

Abstract:

Reactive oxygen species (ROS) produce damage to all major cellular constituents. The antioxidant properties of the ethyl acetate fraction of Empetrum nigrum was assessed against hydrogen peroxide (H(2)O(2))-induced cell damage. Empetrum extract was found to scavenge (1) intracellular ROS in cell system, (2) hydroxyl radicals generated by the Fenton reaction (FeSO(4) + H(2)O(2)), and (3) superoxide radicals generated by xanthine/xanthine oxidase in a cell-free system as detected by electron spin resonance (ESR) spectrometry. Cell damage was produced by H(2)O(2) treatment as evidenced by DNA damage, lipid peroxidation, and increased protein carbonyl formation; however, Empetrum extract prevented H(2)O(2)-induced damage to these parameters. Empetrum extract increased viability of Chinese hamster lung fibroblast (V79-4) cells exposed to H(2)O(2), as evidenced by decreased apoptotic nuclear fragmentation, and lower sub G(1) cell population. Further, Empetrum extract restored the cellular antioxidant enzyme activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), and heme oxygenase-1 (HO-1), which were reduced by H(2)O(2) treatment. In conclusion, Empetrum extract protected cells against H(2)O(2)-induced cell damage via antioxidant properties by scavenging ROS and enhancing antioxidant enzyme activities.

Study Type : In Vitro Study

Print Options


Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.