Effects Of Treatment With Gold Nanoparticles In A Model Of Acute Pulmonary Inflammation Induced By Lipopolysaccharide.
J Biomed Mater Res A. 2019 Sep 10. Epub 2019 Sep 10. PMID: 31502356
Daniela Dos Santos Haupenthal
The bacterial lipopolysaccharide (LPS) is a highly toxic molecule derived from the outer membrane of gram-negative bacteria. LPS endotoxin affects the lungs and is used as a model of acute pulmonary inflammation (IPA) affecting the cellular morphology of the organ. Previously, gold nanoparticles have been shown to demonstrate anti-inflammatory and antioxidative activity in muscle and epithelial injury models. The objective of this study was to investigate the effect of the intraperitoneal treatment using gold nanoparticles (GNPs) on the inflammatory response and pulmonary oxidative stress induced by LPS. Wistar rats were divided into four groups (N = 10): Sham; Sham + GNPs 2.5 mg/kg; LPS; and LPS + GNPs 2.5 mg/kg. Treatment with LPS upregulated the levels of markers of cellular and hepatic damage (CK, LDH, AST, and ALT); however, the group treated with only GNPs exhibited no toxicity. Treatment with GNPs reversed LPS-induced changeswith respect to total peritoneal leukocyte count and the pulmonary levels of pro-inflammatory cytokines (IFN-γ and IL-6). Histological analysis revealed that treatment with GNPs reversed the increase in alveolar septum thickness due to LPS-induced fibrosis. In addition, treatment with GNPs decreased production of oxidants (nitrite and DCFH), reduced oxidative damage (carbonyl and sulfhydryl), and downregulated activities of SOD and CAT. Treatment with GNPs did not showed toxicity; however, it exhibited anti-inflammatory and antioxidative activity that reversed morphological alterations induced by LPS. This article is protected by copyright. All rights reserved.