Hesperidin targets Leishmania donovani Sterol C-24 reductase to fight against Leishmaniasis. - GreenMedInfo Summary
Hesperidin TargetsSterol C-24 Reductase to Fight against Leishmaniasis.
ACS Omega. 2021 Mar 30 ;6(12):8112-8118. Epub 2021 Mar 16. PMID: 33817470
Hesperidin, a naturally occurring flavanoid, is present in citrus family of fruits. It was found effective against an array of pathogens including fungi, bacteria, viruses, and protozoa. Here, we evaluated its antileishmanial activity and possible mechanism of action through differentandexperiments. It inhibited the growth and proliferation of the parasites significantly with a IC50 value of 1.019± 0.116 mM. It also reduced the growth of intra-macrophagic amastigotes with a IC50 value of 0.2858± 0.01398 mM. It induced the reactive oxygen species (ROS) in parasites in a dose-dependent manner. Through 2,7-dichloro dihydro fluorescein diacetate (HDCFDA) staining, it was observed that around 96.9% parasites were ROS positive at 2.0 mM concentration of hesperidin. The ROS generated led to the apoptosis of parasites in a dose-dependent manner as observed by annexin/PI staining. Molecular docking with one of the very important and unique drug-targets ofsterol C-24 reductase resulted in its significant inhibition. Here, we for the first time showed that hesperidin induced the antileishmanial response through the induction of apoptosis and inhibition of sterol C-24 reductase. Our study will be helpful in the development of a cost-effective antileishmanial lead with higher efficacy.