Hexane fraction from Brazilianleaves improved oral carbohydrate tolerance and inhibitsα-amylase and α-glucosidase activities in diabetic mice.
Nat Prod Res. 2020 Feb 24:1-4. Epub 2020 Feb 24. PMID: 32091240
Dionísio Henrique Amaral da Silva
popularly known as mulberry, has been traditionally used as anti-diabetic herbal medication. This study focused on hexane fraction from Brazilianleaves (Hex-Mn) effects on digestion and absorption of carbohydrate in diabetic mice. The high-performance liquid chromatography (HPLC) analysis was performed, and showed the presence of flavonoids isoquercetin and kaempferol-3--rhamnoside. Hex-Mn did not alter oral glucose tolerance test; however, it prevented hyperglycemia in oral sucrose and starch tolerance test in diabetic mice. Also, Hex-Mn was more efficient to inhibit theα-glucosidase, showing lower inhibitory effect on α-amylase activity. The results suggest that Hex-Mn may delay the carbohydrate digestion, but not glucose transport through brush border membrane of the intestine, which contribute with reduction in postprandial hyperglycemia in mice. Hex-Mn has antihyperglycemic effect by attenuating the carbohydrate digestion in diabetic mice, which could be explained, at least in part, by the presence of isoquercetin and kaempferol-3--rhamnoside.