Abstract Title:

Kaempferol stimulates WNT/β-catenin signaling pathway to induce differentiation of osteoblasts.

Abstract Source:

J Nutr Biochem. 2019 Aug 31 ;74:108228. Epub 2019 Aug 31. PMID: 31678747

Abstract Author(s):

Ashish Ranjan Sharma, Ju-Suk Nam

Article Affiliation:

Ashish Ranjan Sharma


Flavonoids, a group of natural compounds found in a variety of vegetables and herbal medicines, have been intensively reported on stimulating bone mineral density and bone formation. Among them, kaempferol has been reported to assist bone formation in vitro and in vivo, but its precise mechanism of action for stimulating bone forming abilities of osteoblasts remained elusive. In SaOS-2 osteoblasts, treatment of kaempferol increased early and late osteogenic parameters significantly, including alkaline phosphatase (ALP) activity, collagen synthesis, and mRNA expression levels of Runx2, osterix, osteopontin and bone sialoprotein. Interestingly, kaempferol promoted osteoblastic differentiation via the activation of the WNT signaling pathway. The stimulation of SaOS-2 cells by kaempferol resulted in an increased activity of WNT signaling responsive reporter construct, Axin-2, and, subsequently, stabilization of WNT signaling mediated transcription factorβ-catenin, probably leading to the activation of WNT-targeted genes for osteogenesis. In corroboration, the kaempferol-induced ALP activity was fully abolished by FH 535, an inhibitor of WNT signaling pathway. Kaempferol mediated activation of WNT signaling pathway through estrogen signaling pathway, as the application of ICI 182,780 (an inhibitor for estrogen receptors) markedly inhibited kaempferol-induced WNT signaling activation and osteogenic marker like ALP activity in SaOS-2 cells. Immunohistochemical studies in drill-hole defect model showed increased expression of Runx2 and β-catenin staining after kaempferol treatment. Thus, it may be concluded that kaempferol stimulates estrogen signaling followed by WNT signaling pathway activation to achieve its potential for bone-sparing effects.

Study Type : Animal Study, In Vitro Study

Print Options

Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2022 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.