The effects of theon the oxidative stress of reproductive system in diabetic male rats.
Int J Reprod Biomed (Yazd). 2019 Jul ;17(7):493-502. Epub 2019 Jul 31. PMID: 31508575
Seddigheh Sheikh Hosseini
Background: Oxidative stress plays an important role in the development of diabetic complications.
Objective: This study evaluated the impact of pre- and post-treatment withon the oxidant and anti-oxidant factors of testis and epididymis in streptozotocin-induced diabetic rats.
Materials and Methods: Thirty male Wistar rats (10 wk old) weighing 220-230 g. were divided into five groups (n = 6/ each): 1- normal group, 2- normalgroup, 3- diabetic group, 4- diabetic +before (DLB) group, and 5- diabetic +(DLA) group. The normal and diabetic groups received daily 1 mL normal saline for 6 wk. Normalgroup received dailyfor 6 wk. Group DLB received dailyfor 2 wk before diabetes and for 4 wk after diabetes. Group DLA received daily 1 mL normal saline for 2 wk before diabetes andfor 4 wk after diabetes. The dose ofwas 110CFU/mL.
Results: The administration ofworsened blood glucose level and reduced the levels of Malondialdehyde (p0.0001) and Hydrogen peroxide (p0.0001) and, Catalase and Glutathione peroxidase activity increased in the testis. In epididymis, Glutathione peroxidase and Catalase (p = 0.013) activity increased and Hydrogen peroxide concentration reduced, while Malondialdehyde concentration did not show any changes compared to the diabetic rats. Also, there was no significant difference between DLB and DLA groups, in these markers.
Conclusion: Data obtained suggests thathas anti-oxidant effects on the testis and sometime in the epididymis in diabetic rats.