Pecan pericarp extract protects against carbon tetrachloride-induced liver injury through oxidative mechanism in rats.
Toxicol Res (Camb). 2020 Sep ;9(5):652-660. Epub 2020 Sep 24. PMID: 33178425
The purpose of this study was to quantify the proanthocyanidin content of pecan () pericarp extract (PPE) and to assess its useful impacts against carbon tetrachloride (CCl)-induced hepatotoxicity. Rats were randomly divided into four groups: Group 1: received intraperitoneal injection of saline solution, Group 2: was injected with PPE (25 mg/kg body weight) for 10 consecutive days, Group 3: received CCl(0.5 ml/kg, subcutaneous injection), Group 4: was coadministred with PPE + CCl. The CClwas administered every 3 days during 10 days. Results revealed the presence of a high amount of total proanthocyanidins in the PPE (81.01 ± 0.21 mg TAE.gDW). CClinjection induced significant reductions in hepatic antioxidants but increased hepatic lipid peroxidation (LPO) as well as serum injury biomarkers. However, cotreatment with PPE significantly ( < 0.05) inverted CCl-induced increase in plasma alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and lactate dehydrogenase activities, respectively to 74%, 77%, 60%, and 82% compared with CClgroup. No significant toxic effects were observed following treatment with plant extract alone. PPE cotreatment also decreased significant ( < 0.05) the hepatic malondialdehyde formation (21%) and enhanced the liver catalase activity (107%) in CCl-intoxicated rats. The histopathological examination showed inflammatory infiltration and degenerative changes in the hepatic tissue following CClinjection. The hepatoprotective activity of PPE against CClexposure was supported by the maintenance of structural integrity of liver histopathology. In conclusion, the current study illustrated that PPE pretreatment significantly improved all examined parameters, restored the hepatic architecture and successfully alleviates oxidative damage induced by CClintoxication.