Synergistic activity of melittin with mupirocin against methicillin-resistant S. Aureus. - GreenMedInfo Summary
Synergistic activity of melittin with mupirocin: A study against methicillin-resistant(MRSA) and methicillin-susceptible(MSSA) isolates.
Saudi J Biol Sci. 2020 Oct ;27(10):2580-2585. Epub 2020 May 23. PMID: 32994714
Reza Hakimi Alni
Methicillin-Resistant(MRSA) biofilms are involved in various nosocomial infections, being in the limelight of academic research. The current study aimed to determine the antimicrobial effects of melittin on planktonic and biofilm forms of. Following the identification of MRSA and SCCmec types (using PCR method), Minimum Inhibitory Concentration (MIC), Minimum Bactericidal Concentration (MBC), and fractional inhibitory concentration index (FICi), for melittin and mupirocin were determined by broth microdilution assay. Melittin anti-biofilm activity was determined, using a microtiter-plate test (MtP) and scanning electron microscope (SEM) methods. The quorum sensing inhibitory activity of½ MIC melittin was examined using a quantitative real-time RT-PCR method, and melittin cytotoxicity on Vero cells was examined by tetrazolium-based colorimetric (MTT) test. The Results of our study showed that Geometric means of MIC values of the melittin and mupirocin were 4.4 and 14.22 μg/ml respectively. The geometric mean of the FICi for both melittin-mupirocin was 0.75. Nobiofilm was formed andgene (as a biofilm regulator) expression down-regulated. It seems that melittin can be useful in the treatment ofinfections (especially MRSA) by reducing theexpression. Furthermore, synergistic growth-inhibitory effects of mupirocin with melittin could be considered as a promising approach in the treatment of MRSA isolates.