Abstract Title:

Tooth brushing using toothpaste containing theaflavins reduces the oral pathogenic bacteria in healthy adults.

Abstract Source:

3 Biotech. 2021 Mar ;11(3):150. Epub 2021 Mar 2. PMID: 33747700

Abstract Author(s):

Junhao Kong, Guoqing Zhang, Kai Xia, Chunhua Diao, Xiufang Yang, Xiaobo Zuo, Yudong Li, Xinle Liang

Article Affiliation:

Junhao Kong


: Theaflavins (TFs) are the main bioactive component in black tea. At present, little effort has been done to evaluate the influence of TFs when included in the toothpaste on the diversity of oral microbiota. In this study, eighty samples collected from the saliva and supragingival plaque of 20 healthy adults using toothpaste with the absence or presence of TFs for a period of 4 weeks were used for the oral microbiome analysis by 16S rRNA gene sequencing. Alpha and beta diversity analysis showed that tooth brushing using the toothpaste with TFs significantly increased the microbial abundance in the saliva samples, and altered the oral microbiota obtained from the saliva and supragingival plaque. The linear discriminant analysis revealed that the use of toothpaste with TFs significantly reduced the abundance of oral pathogens (e.g.,,, and) while increased the abundance of oral-health associated bacteria (e.g.,and). In addition, using toothpaste with TFs reduced the functional pathways abundance relevance to the extracellular polymeric substance (EPS) synthesis while enriched the functions in transporters, ABC transporters, two-component system, and amino acid metabolism. Collectively, our results provide evidence for the application of toothpaste containing TFs as a promising oral care product.

Supplementary Information: The online version contains supplementary material available at 10.1007/s13205-021-02699-7.

Study Type : Human Study

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