Bioss: lethal effects of methanolic extract against protoscoleces of.
J Parasit Dis. 2016 Dec ;40(4):1289-1292. Epub 2015 Mar 14. PMID: 27876932
The present study was aimed to evaluate the lethal effects ofBoiss (Lamiaceae) methanolic extract againstprotoscoleces. Protoscoleces were aseptically aspirated from sheep livers having hydatid cysts. Various concentrations of the essential oil (2.5-20 mg/mL) were used for 10-60 min. Viability of protoscoleces was confirmed using eosin exclusion test (0.1 % eosin staining). Obtained results showed thatextract at the concentration of 20 mg/mL after 10 min of exposure killed 100 % protoscoleces. The mean of mortality rate of protoscoleces after 20 min of exposure to the concentration of 10 mg/mL was also 100 %. Lower concentrations ofextract provoked a delayed protoscolicidal activity. The findings indicated potential ofmethanolic extract as a natural source for the producing of new scolicidal agent for use in hydatid cyst surgery.