n/a
Article Publish Status: FREE
Abstract Title:

Effect of Sub-Minimum Inhibitory Concentrations of Tyrosol and EDTA on Quorum Sensing and Virulence of.

Abstract Source:

Infect Drug Resist. 2020 ;13:3501-3511. Epub 2020 Oct 8. PMID: 33116669

Abstract Author(s):

Shaymaa H Abdel-Rhman, Dina E Rizk, Eman S Abdelmegeed

Article Affiliation:

Shaymaa H Abdel-Rhman

Abstract:

Introduction: is considered a dangerous pathogen, as it causes many human diseases, besides that it is resistant to almost all types of antibacterial agents. So, new strategies to overcomeinfection have evolved to attenuate its virulence factors and inhibit its quorum-sensing (QS) activity.

Purpose: This study investigated the effect of tyrosol and EDTA as anti-quorum-sensing and antivirulence agents againstPAO1.

Methods: Anti-quorum activity of sub-minimum inhibitory concentrations (sub-MICs) of tyrosol and EDTA was tested using(CV 12,472) biosensor bioassay. Miller assay was used to assess the inhibition of QS signal molecules byβ-galactosidase activity determination. Also, their effects on the production of protease, lipase, lecithinase, and motility were tested. The inhibitory effects of these molecules on QS regulatory genes and exotoxins genes expression were evaluated by real-time PCR.

Results: Tyrosol and EDTA at sub-MICs inhibited the production of violacein pigment. Both compounds inhibited QS molecules production and their associated virulence factors (protease, lipase, lecithinase, and motility) (P≤ 0.05). Besides, the expression levels of QS regulatory genes (and) and exotoxins genes () were significantly reduced (P≤ 0.05).

Conclusion: Both tyrosol and EDTA can be used to fightinfection as anti-quorum-sensing and antivirulence agents at their sub-MICs.

Study Type : In Vitro Study

Print Options


Key Research Topics

Sayer Ji
Founder of GreenMedInfo.com

Subscribe to our informative Newsletter & get Nature's Evidence-Based Pharmacy

Our newsletter serves 500,000 with essential news, research & healthy tips, daily.

Download Now

500+ pages of Natural Medicine Alternatives and Information.

This website is for information purposes only. By providing the information contained herein we are not diagnosing, treating, curing, mitigating, or preventing any type of disease or medical condition. Before beginning any type of natural, integrative or conventional treatment regimen, it is advisable to seek the advice of a licensed healthcare professional.

© Copyright 2008-2021 GreenMedInfo.com, Journal Articles copyright of original owners, MeSH copyright NLM.