Abstract Title:

Effect of PVP on sperm capacitation status and embryonic development in cattle.

Abstract Source:

Theriogenology. 2009 Sep 15;72(5):624-35. Epub 2009 Jul 14. PMID: 19604569

Abstract Author(s):

Y Kato, Y Nagao

Article Affiliation:

Department of Animal Production Science, United Graduate School of Agricultural Science, Tokyo University of Agriculture and Technology, Fuchu, 183-8509, Japan.


The objective of this study was to investigate the effects of polyvinylpyrrolidone (PVP) on sperm capacitation status and embryonic development in cattle (Bos taurus). Acrosome-reacted sperm (chlortetracycline stain) and the fertilization rate after intracytoplasmic sperm injection (ICSI) were enhanced (P<0.05) when sperm were cultured in a medium containing 10% PVP. However, injection of bovine in vitro-produced (IVP) embryos with medium containing 10% PVP suppressed development of these embryos to the 2-cell, morula, and blastocyst stages and cell number at the blastocyst stage (P<0.01) but did not affect chromosomal integrity (P>0.05). Embryo developmental capacity differed (P<0.01) among PVP from three suppliers, but there were no significant differences among three solvent media. The PVP remained localized in 40.9% of PVP-injected IVP embryos. In conclusion, PVP affected the acrosome reaction and enhanced the fertilization rate after ICSI. However, PVP remained detectable in IVP embryos and suppressed their developmental capacity.

Study Type : Animal Study
Additional Links
Problem Substances : Crospovidone : CK(2) : AC(1)

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