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Article Publish Status: FREE
Abstract Title:

In Vivo and In Vitro Hepatoprotective Effects of Geranium koreanum Methanolic Extract via Downregulation of MAPK/Caspase-3 Pathway.

Abstract Source:

Evid Based Complement Alternat Med. 2017 ;2017:8137627. Epub 2017 Jul 5. PMID: 28757890

Abstract Author(s):

Md Rashedunnabi Akanda, In-Shik Kim, Dongchoon Ahn, Hyun-Jin Tae, Weishun Tian, Hyeon-Hwa Nam, Byung-Kil Choo, Byung-Yong Park

Article Affiliation:

Md Rashedunnabi Akanda

Abstract:

Geranium koreanum (GK) is an indigenous Chinese herbal medicine widely used for the treatment of various inflammation and liver disorders. However, the exact mechanism of action of GK remains unknown. This study aimed to investigate the protective effect and related molecular mechanism of GK on NaAsO2-induced cytotoxicity in HepG2 cells and liver damage in mice. The cytoprotective role of GK was assessed on HepG2 cells using MTT assay. Oxidative stress and lactate dehydrogenase levels were measured with ROS and LDH assay. Histopathology and serum enzymes levels were estimated. The molecular mechanism was evaluated by qPCR and immunoblotting to ensure the hepatoprotective role of GK against NaAsO2 intoxication in mice. We found cotreatment with GK significantly attenuated NaAsO2-induced cell viability loss, intracellular ROS, and LDH release. Hepatic histopathology and serum biochemical parameters, ALT, and AST were notably improved by cotreatment with GK. Beside, GK markedly altered both mRNA and protein expression level of MAPK. The proapoptotic and antiapoptotic protein Bax/Bcl-2 ratio was significantly regulated by GK. Moreover, GK remarkably suppressed the postapoptotic transcription protein cleaved caspase-3 expression. The present study reveals that GK possesses hepatoprotective activity which is probably involved in the modulation of the MAPK/caspase-3 pathway.

Study Type : Animal Study, In Vitro Study
Additional Links
Pharmacological Actions : Hepatoprotective : CK(5098) : AC(2264)

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